Thin Sections

Thin Sections

Knowledge of the thickness of sections is important for proper interpretation of electron micrographs. Therefore, the thicknesses of sections of n-butyl methacrylate polymer were determined by ellipsometry, and correlated with the color shown in reflected light. The results are: gray, thinner than 60 mmicro; silver, 60 to 90 mmicro; gold, 90 to 150 mmicro; purple, 150 to 190 mmicro; blue, 190 t...

Educational Meteorite Thin Sections

Routine Preparation of Potished Thin-sections

The colour plate which appears in this number of the Canadian Mineralogist iliustrates the advantage of microscopic observation of mineralized rocks in polished thin-section by incident and transmitted cross-polarized light simultaneously: the identities and relationships of opuqrr" and translucent minerals in contact are immediately evident. The principle is not ne.w; more than a hundred years...

Preparation of thin epoxy resin sections from thick sections of paraffin-embedded material.

PROCEDURES The following have been used with success: haematoxylin and eosin, Perls's reaction for ferric iron, the periodic acid-Schiff reaction for carbohydrates, the alcian blue method for acid mucopolysaccharides, the Feulgen reaction for desoxyribonucleic acid, the periodic acid-hexamine silver method, Luxol fast blue for myelin, solochrome cyanine as a general stain, Heidenhain's iron hae...

A Method of Staining Thin Sections with Lead Hydroxide for Precipitate-free Sections

Staining of thin sections results in greater contrast which facilitates focusing and the observation of fine structure. Lead hydroxide, as suggested by Watson (1), has become increasingly popular as a stain for electron microscopy. However, when lead hydroxide is exposed to the carbon dioxide of air, lead carbonate forms and is deposited on the sections as fine, needle-like crystals, large amor...